Dr. rer. nat. Stephanie Daniela Wolf

Pic of Dr. rer. nat. Stephanie Daniela Wolf

Postdoc - Department of Gastroenterology, Hepatology and Infectious Disease - University Hospital Düsseldorf

Role in LiSyM-Cancer

  • Collection, processing, and biobanking of clinical patient samples and associated clinical data
  • Development and application of mouse models for MASLD, MASH, and hepatocellular carcinoma (HCC)
  • Investigation of the roles of PAR4, MK2, and MK3 in liver disease progression
  • Characterization of macrophage subpopulation dynamics during the progression from MASLD to HCC
  • Analysis of the role of TGF-β signaling in macrophage-mediated disease progression
  • Investigation of intercellular communication between macrophages and hepatocytes during liver disease progression
  • Supervisor: Prof. Johannes G. Bode (P07)

Expertise

  • Isolation of different liver cell types (Hepatocytes, non-parenchymal cells), mouse and human
  • Cell-cell communication: co-cultivation of primary cells
  • Liver injury and regeneration: partial hepatectomy
  • MASLD disease progression / HCC
  • Preclinical mouse models, animal experimentation, and biobanking
  • Molecular biology, flow cytometry, CITE-seq analysis, IHC, IF
  • Immunology, inflammation, Macrophages
  • Data Analysis
  • Coordination of research alliances and network management
  • Scientific Project Management

Professional Experience

  • Reseach project/networks:
    • Virtual liver network (BMFTR, 2011 – 2015)
    • SFB974: "Communication and Systemic Relevance in Liver Injury and Regeneration" (DFG, 2015 – 2021)
    • LiSyM-Krebs I / II (BMFTR, 2021 – 2026)
    • Characterization of the cell type-specific role of protease-activated receptor 4 (PAR4) in MASLD pathogenesis and disease progression (DFG, since 2026)

Degrees

  • PhD (Dr. rer. nat.)
    Faculty of Medicine & Düsseldorf University Hospital, Heinrich Heine University
    Department of Gastroenterology, Hepatology and Infectious Disease:
    Identification of differentially regulated mediators during liver regeneration and investigation of the reciprocal modulation of macrophage and hepatocyte function through intercellular communication.

  • Diploma thesis
    Ernst-Moritz-Arndt University, Greifswald Department of Pharmacology, Center for Pharmacology and Experimental Therapeutics
    Functional characterization of the transporter SLC2A9

Honors and Awards

  • Poster Award, German Association for the Study of the Liver Annual Meeting, for the poster "Interference of TGFb with the activation state of liver macrophages and consequences for liver injury and regeneration."
  • Poster Award, Experimental Hepatology Days, for the poster "Changes in macrophage subpopulations towards a recruited, TGFb-sensitive population accompany progression of NASH“